This bingo card has a free space and 24 words: (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), List two reasons why numerical aperture is important for fluorescence microscopy., Photobleaching occurs by ______ state fluorophores reacting with ________., List one way fluorescent proteins are better than quantum dots, and one way they are not., In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, List one advantage and one disadvantage of small organic dyes., Arc lamps pass electricity through ______, _______, or _______ vapour, Quantum dots can be approximated as _______., A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, Light passes the objective lens ______ before reaching the observer or camera., List a database where information on fluorophores can be found., List one benefit of using a deep neural net to reconstruct images., Small organic dyes only work with ______, ______ cells., When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., List the types of fluorescent markers from smallest to largest. and What was the first chemical to be known to have fluorescent properties, and who discovered this fact?.
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