This bingo card has a free space and 24 words: Quantum dots can be approximated as _______., Arc lamps pass electricity through ______, _______, or _______ vapour, List two reasons why numerical aperture is important for fluorescence microscopy., List two ways photobleaching can be slowed down., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., Light passes the objective lens ______ before reaching the observer or camera., Small organic dyes only work with ______, ______ cells., List one advantage and one disadvantage of small organic dyes., (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample., Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., List one way fluorescent proteins are better than quantum dots, and one way they are not., List one benefit of using a deep neural net to reconstruct images., List the types of fluorescent markers from smallest to largest., How should light sources for fluorescence microscopy be chosen?, DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, Photobleaching occurs by ______ state fluorophores reacting with ________., FlAsH and ReAsH are used in conjunction with _______ proteins., What colour wavelength is the hardest to directly engineer from a green fluorescent protein? and Dichroic mirrors reflect _____ wavelengths and transmit ______ ones..
Wave Revision | Life Science Chpt 2 Cells | Chemistry Review | Radiation Physics | Chapter 8 Review
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