WrongprimersRan gelbackwardsBand inthenegativecontrolWrongannealingtempUsed proteinmarker asDNA ladderForgotmarkerToo shortelongationtimeForgot toturn thegel onNo bandin thepositivecontrolMultipleannealingsitesPreparedagarosegel withdH2OFrameshiftmutationForgotgelstainDidn't mixwithsamplebufferRan geltoolongNotenoughmastermixWrongsalt/bufferconcentrationForgottemplateDidn'tmixproperlyToo highvoltage,gelliquifiedSamplespilloverinto nextlanePrimerinsertionSpilledthesampleWrongPCRprogramWrongprimersRan gelbackwardsBand inthenegativecontrolWrongannealingtempUsed proteinmarker asDNA ladderForgotmarkerToo shortelongationtimeForgot toturn thegel onNo bandin thepositivecontrolMultipleannealingsitesPreparedagarosegel withdH2OFrameshiftmutationForgotgelstainDidn't mixwithsamplebufferRan geltoolongNotenoughmastermixWrongsalt/bufferconcentrationForgottemplateDidn'tmixproperlyToo highvoltage,gelliquifiedSamplespilloverinto nextlanePrimerinsertionSpilledthesampleWrongPCRprogram

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Wrong primers
  2. Ran gel backwards
  3. Band in the negative control
  4. Wrong annealing temp
  5. Used protein marker as DNA ladder
  6. Forgot marker
  7. Too short elongation time
  8. Forgot to turn the gel on
  9. No band in the positive control
  10. Multiple annealing sites
  11. Prepared agarose gel with dH2O
  12. Frameshift mutation
  13. Forgot gel stain
  14. Didn't mix with sample buffer
  15. Ran gel too long
  16. Not enough mastermix
  17. Wrong salt/buffer concentration
  18. Forgot template
  19. Didn't mix properly
  20. Too high voltage, gel liquified
  21. Sample spillover into next lane
  22. Primer insertion
  23. Spilled the sample
  24. Wrong PCR program