No bandin thepositivecontrolWrongannealingtempForgotmarkerDidn't mixwithsamplebufferWrongprimersWrongPCRprogramBand inthenegativecontrolUsed proteinmarker asDNA ladderMultipleannealingsitesSpilledthesampleForgottemplateForgot toturn thegel onToo highvoltage,gelliquifiedNotenoughmastermixPreparedagarosegel withdH2ORan gelbackwardsRan geltoolongPrimerinsertionForgotgelstainWrongsalt/bufferconcentrationFrameshiftmutationDidn'tmixproperlySamplespilloverinto nextlaneToo shortelongationtimeNo bandin thepositivecontrolWrongannealingtempForgotmarkerDidn't mixwithsamplebufferWrongprimersWrongPCRprogramBand inthenegativecontrolUsed proteinmarker asDNA ladderMultipleannealingsitesSpilledthesampleForgottemplateForgot toturn thegel onToo highvoltage,gelliquifiedNotenoughmastermixPreparedagarosegel withdH2ORan gelbackwardsRan geltoolongPrimerinsertionForgotgelstainWrongsalt/bufferconcentrationFrameshiftmutationDidn'tmixproperlySamplespilloverinto nextlaneToo shortelongationtime

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. No band in the positive control
  2. Wrong annealing temp
  3. Forgot marker
  4. Didn't mix with sample buffer
  5. Wrong primers
  6. Wrong PCR program
  7. Band in the negative control
  8. Used protein marker as DNA ladder
  9. Multiple annealing sites
  10. Spilled the sample
  11. Forgot template
  12. Forgot to turn the gel on
  13. Too high voltage, gel liquified
  14. Not enough mastermix
  15. Prepared agarose gel with dH2O
  16. Ran gel backwards
  17. Ran gel too long
  18. Primer insertion
  19. Forgot gel stain
  20. Wrong salt/buffer concentration
  21. Frameshift mutation
  22. Didn't mix properly
  23. Sample spillover into next lane
  24. Too short elongation time