Band inthenegativecontrolRan geltoolongUsed proteinmarker asDNA ladderSpilledthesampleForgot toturn thegel onMultipleannealingsitesFrameshiftmutationPrimerinsertionDidn'tmixproperlyRan gelbackwardsToo shortelongationtimeDidn't mixwithsamplebufferPreparedagarosegel withdH2OWrongannealingtempForgottemplateWrongsalt/bufferconcentrationWrongPCRprogramToo highvoltage,gelliquifiedWrongprimersNotenoughmastermixSamplespilloverinto nextlaneForgotgelstainForgotmarkerNo bandin thepositivecontrolBand inthenegativecontrolRan geltoolongUsed proteinmarker asDNA ladderSpilledthesampleForgot toturn thegel onMultipleannealingsitesFrameshiftmutationPrimerinsertionDidn'tmixproperlyRan gelbackwardsToo shortelongationtimeDidn't mixwithsamplebufferPreparedagarosegel withdH2OWrongannealingtempForgottemplateWrongsalt/bufferconcentrationWrongPCRprogramToo highvoltage,gelliquifiedWrongprimersNotenoughmastermixSamplespilloverinto nextlaneForgotgelstainForgotmarkerNo bandin thepositivecontrol

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Band in the negative control
  2. Ran gel too long
  3. Used protein marker as DNA ladder
  4. Spilled the sample
  5. Forgot to turn the gel on
  6. Multiple annealing sites
  7. Frameshift mutation
  8. Primer insertion
  9. Didn't mix properly
  10. Ran gel backwards
  11. Too short elongation time
  12. Didn't mix with sample buffer
  13. Prepared agarose gel with dH2O
  14. Wrong annealing temp
  15. Forgot template
  16. Wrong salt/buffer concentration
  17. Wrong PCR program
  18. Too high voltage, gel liquified
  19. Wrong primers
  20. Not enough mastermix
  21. Sample spillover into next lane
  22. Forgot gel stain
  23. Forgot marker
  24. No band in the positive control