Preparedagarosegel withdH2OWrongsalt/bufferconcentrationPrimerinsertionWrongPCRprogramForgotgelstainWrongprimersDidn't mixwithsamplebufferForgottemplateNotenoughmastermixNo bandin thepositivecontrolDidn'tmixproperlyUsed proteinmarker asDNA ladderBand inthenegativecontrolWrongannealingtempToo highvoltage,gelliquifiedFrameshiftmutationRan geltoolongMultipleannealingsitesForgotmarkerForgot toturn thegel onSamplespilloverinto nextlaneToo shortelongationtimeRan gelbackwardsSpilledthesamplePreparedagarosegel withdH2OWrongsalt/bufferconcentrationPrimerinsertionWrongPCRprogramForgotgelstainWrongprimersDidn't mixwithsamplebufferForgottemplateNotenoughmastermixNo bandin thepositivecontrolDidn'tmixproperlyUsed proteinmarker asDNA ladderBand inthenegativecontrolWrongannealingtempToo highvoltage,gelliquifiedFrameshiftmutationRan geltoolongMultipleannealingsitesForgotmarkerForgot toturn thegel onSamplespilloverinto nextlaneToo shortelongationtimeRan gelbackwardsSpilledthesample

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Prepared agarose gel with dH2O
  2. Wrong salt/buffer concentration
  3. Primer insertion
  4. Wrong PCR program
  5. Forgot gel stain
  6. Wrong primers
  7. Didn't mix with sample buffer
  8. Forgot template
  9. Not enough mastermix
  10. No band in the positive control
  11. Didn't mix properly
  12. Used protein marker as DNA ladder
  13. Band in the negative control
  14. Wrong annealing temp
  15. Too high voltage, gel liquified
  16. Frameshift mutation
  17. Ran gel too long
  18. Multiple annealing sites
  19. Forgot marker
  20. Forgot to turn the gel on
  21. Sample spillover into next lane
  22. Too short elongation time
  23. Ran gel backwards
  24. Spilled the sample