WrongPCRprogramForgotgelstainForgot toturn thegel onSpilledthesampleNotenoughmastermixToo highvoltage,gelliquifiedFrameshiftmutationToo shortelongationtimePrimerinsertionForgottemplateWrongannealingtempWrongsalt/bufferconcentrationNo bandin thepositivecontrolSamplespilloverinto nextlaneDidn'tmixproperlyRan geltoolongDidn't mixwithsamplebufferPreparedagarosegel withdH2OForgotmarkerMultipleannealingsitesRan gelbackwardsUsed proteinmarker asDNA ladderWrongprimersBand inthenegativecontrolWrongPCRprogramForgotgelstainForgot toturn thegel onSpilledthesampleNotenoughmastermixToo highvoltage,gelliquifiedFrameshiftmutationToo shortelongationtimePrimerinsertionForgottemplateWrongannealingtempWrongsalt/bufferconcentrationNo bandin thepositivecontrolSamplespilloverinto nextlaneDidn'tmixproperlyRan geltoolongDidn't mixwithsamplebufferPreparedagarosegel withdH2OForgotmarkerMultipleannealingsitesRan gelbackwardsUsed proteinmarker asDNA ladderWrongprimersBand inthenegativecontrol

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Wrong PCR program
  2. Forgot gel stain
  3. Forgot to turn the gel on
  4. Spilled the sample
  5. Not enough mastermix
  6. Too high voltage, gel liquified
  7. Frameshift mutation
  8. Too short elongation time
  9. Primer insertion
  10. Forgot template
  11. Wrong annealing temp
  12. Wrong salt/buffer concentration
  13. No band in the positive control
  14. Sample spillover into next lane
  15. Didn't mix properly
  16. Ran gel too long
  17. Didn't mix with sample buffer
  18. Prepared agarose gel with dH2O
  19. Forgot marker
  20. Multiple annealing sites
  21. Ran gel backwards
  22. Used protein marker as DNA ladder
  23. Wrong primers
  24. Band in the negative control