PrimerinsertionUsed proteinmarker asDNA ladderFrameshiftmutationForgottemplateForgotgelstainPreparedagarosegel withdH2ONotenoughmastermixDidn't mixwithsamplebufferWrongannealingtempMultipleannealingsitesRan geltoolongWrongPCRprogramWrongsalt/bufferconcentrationToo highvoltage,gelliquifiedToo shortelongationtimeForgot toturn thegel onForgotmarkerRan gelbackwardsSamplespilloverinto nextlaneNo bandin thepositivecontrolBand inthenegativecontrolWrongprimersDidn'tmixproperlySpilledthesamplePrimerinsertionUsed proteinmarker asDNA ladderFrameshiftmutationForgottemplateForgotgelstainPreparedagarosegel withdH2ONotenoughmastermixDidn't mixwithsamplebufferWrongannealingtempMultipleannealingsitesRan geltoolongWrongPCRprogramWrongsalt/bufferconcentrationToo highvoltage,gelliquifiedToo shortelongationtimeForgot toturn thegel onForgotmarkerRan gelbackwardsSamplespilloverinto nextlaneNo bandin thepositivecontrolBand inthenegativecontrolWrongprimersDidn'tmixproperlySpilledthesample

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Primer insertion
  2. Used protein marker as DNA ladder
  3. Frameshift mutation
  4. Forgot template
  5. Forgot gel stain
  6. Prepared agarose gel with dH2O
  7. Not enough mastermix
  8. Didn't mix with sample buffer
  9. Wrong annealing temp
  10. Multiple annealing sites
  11. Ran gel too long
  12. Wrong PCR program
  13. Wrong salt/buffer concentration
  14. Too high voltage, gel liquified
  15. Too short elongation time
  16. Forgot to turn the gel on
  17. Forgot marker
  18. Ran gel backwards
  19. Sample spillover into next lane
  20. No band in the positive control
  21. Band in the negative control
  22. Wrong primers
  23. Didn't mix properly
  24. Spilled the sample