Samplespilloverinto nextlaneForgotgelstainWrongprimersForgot toturn thegel onWrongPCRprogramWrongsalt/bufferconcentrationForgotmarkerFrameshiftmutationToo highvoltage,gelliquifiedRan geltoolongDidn'tmixproperlyToo shortelongationtimeBand inthenegativecontrolNo bandin thepositivecontrolMultipleannealingsitesPrimerinsertionUsed proteinmarker asDNA ladderWrongannealingtempForgottemplateNotenoughmastermixPreparedagarosegel withdH2ODidn't mixwithsamplebufferSpilledthesampleRan gelbackwardsSamplespilloverinto nextlaneForgotgelstainWrongprimersForgot toturn thegel onWrongPCRprogramWrongsalt/bufferconcentrationForgotmarkerFrameshiftmutationToo highvoltage,gelliquifiedRan geltoolongDidn'tmixproperlyToo shortelongationtimeBand inthenegativecontrolNo bandin thepositivecontrolMultipleannealingsitesPrimerinsertionUsed proteinmarker asDNA ladderWrongannealingtempForgottemplateNotenoughmastermixPreparedagarosegel withdH2ODidn't mixwithsamplebufferSpilledthesampleRan gelbackwards

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Sample spillover into next lane
  2. Forgot gel stain
  3. Wrong primers
  4. Forgot to turn the gel on
  5. Wrong PCR program
  6. Wrong salt/buffer concentration
  7. Forgot marker
  8. Frameshift mutation
  9. Too high voltage, gel liquified
  10. Ran gel too long
  11. Didn't mix properly
  12. Too short elongation time
  13. Band in the negative control
  14. No band in the positive control
  15. Multiple annealing sites
  16. Primer insertion
  17. Used protein marker as DNA ladder
  18. Wrong annealing temp
  19. Forgot template
  20. Not enough mastermix
  21. Prepared agarose gel with dH2O
  22. Didn't mix with sample buffer
  23. Spilled the sample
  24. Ran gel backwards