PrimerinsertionSamplespilloverinto nextlaneToo highvoltage,gelliquifiedNo bandin thepositivecontrolToo shortelongationtimeForgotgelstainRan gelbackwardsPreparedagarosegel withdH2ONotenoughmastermixForgottemplateBand inthenegativecontrolWrongPCRprogramDidn't mixwithsamplebufferUsed proteinmarker asDNA ladderMultipleannealingsitesForgot toturn thegel onWrongprimersSpilledthesampleFrameshiftmutationDidn'tmixproperlyWrongannealingtempRan geltoolongForgotmarkerWrongsalt/bufferconcentrationPrimerinsertionSamplespilloverinto nextlaneToo highvoltage,gelliquifiedNo bandin thepositivecontrolToo shortelongationtimeForgotgelstainRan gelbackwardsPreparedagarosegel withdH2ONotenoughmastermixForgottemplateBand inthenegativecontrolWrongPCRprogramDidn't mixwithsamplebufferUsed proteinmarker asDNA ladderMultipleannealingsitesForgot toturn thegel onWrongprimersSpilledthesampleFrameshiftmutationDidn'tmixproperlyWrongannealingtempRan geltoolongForgotmarkerWrongsalt/bufferconcentration

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Primer insertion
  2. Sample spillover into next lane
  3. Too high voltage, gel liquified
  4. No band in the positive control
  5. Too short elongation time
  6. Forgot gel stain
  7. Ran gel backwards
  8. Prepared agarose gel with dH2O
  9. Not enough mastermix
  10. Forgot template
  11. Band in the negative control
  12. Wrong PCR program
  13. Didn't mix with sample buffer
  14. Used protein marker as DNA ladder
  15. Multiple annealing sites
  16. Forgot to turn the gel on
  17. Wrong primers
  18. Spilled the sample
  19. Frameshift mutation
  20. Didn't mix properly
  21. Wrong annealing temp
  22. Ran gel too long
  23. Forgot marker
  24. Wrong salt/buffer concentration