Used proteinmarker asDNA ladderBand inthenegativecontrolWrongsalt/bufferconcentrationRan geltoolongForgotgelstainDidn't mixwithsamplebufferWrongPCRprogramSpilledthesampleWrongannealingtempToo shortelongationtimeDidn'tmixproperlyPreparedagarosegel withdH2OForgot toturn thegel onWrongprimersSamplespilloverinto nextlaneNotenoughmastermixPrimerinsertionMultipleannealingsitesNo bandin thepositivecontrolToo highvoltage,gelliquifiedForgottemplateRan gelbackwardsFrameshiftmutationForgotmarkerUsed proteinmarker asDNA ladderBand inthenegativecontrolWrongsalt/bufferconcentrationRan geltoolongForgotgelstainDidn't mixwithsamplebufferWrongPCRprogramSpilledthesampleWrongannealingtempToo shortelongationtimeDidn'tmixproperlyPreparedagarosegel withdH2OForgot toturn thegel onWrongprimersSamplespilloverinto nextlaneNotenoughmastermixPrimerinsertionMultipleannealingsitesNo bandin thepositivecontrolToo highvoltage,gelliquifiedForgottemplateRan gelbackwardsFrameshiftmutationForgotmarker

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Used protein marker as DNA ladder
  2. Band in the negative control
  3. Wrong salt/buffer concentration
  4. Ran gel too long
  5. Forgot gel stain
  6. Didn't mix with sample buffer
  7. Wrong PCR program
  8. Spilled the sample
  9. Wrong annealing temp
  10. Too short elongation time
  11. Didn't mix properly
  12. Prepared agarose gel with dH2O
  13. Forgot to turn the gel on
  14. Wrong primers
  15. Sample spillover into next lane
  16. Not enough mastermix
  17. Primer insertion
  18. Multiple annealing sites
  19. No band in the positive control
  20. Too high voltage, gel liquified
  21. Forgot template
  22. Ran gel backwards
  23. Frameshift mutation
  24. Forgot marker