Too highvoltage,gelliquifiedDidn't mixwithsamplebufferFrameshiftmutationForgot toturn thegel onToo shortelongationtimeForgottemplateMultipleannealingsitesForgotmarkerDidn'tmixproperlyNo bandin thepositivecontrolWrongsalt/bufferconcentrationPrimerinsertionRan gelbackwardsRan geltoolongPreparedagarosegel withdH2OBand inthenegativecontrolWrongannealingtempWrongprimersForgotgelstainWrongPCRprogramUsed proteinmarker asDNA ladderSpilledthesampleNotenoughmastermixSamplespilloverinto nextlaneToo highvoltage,gelliquifiedDidn't mixwithsamplebufferFrameshiftmutationForgot toturn thegel onToo shortelongationtimeForgottemplateMultipleannealingsitesForgotmarkerDidn'tmixproperlyNo bandin thepositivecontrolWrongsalt/bufferconcentrationPrimerinsertionRan gelbackwardsRan geltoolongPreparedagarosegel withdH2OBand inthenegativecontrolWrongannealingtempWrongprimersForgotgelstainWrongPCRprogramUsed proteinmarker asDNA ladderSpilledthesampleNotenoughmastermixSamplespilloverinto nextlane

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Too high voltage, gel liquified
  2. Didn't mix with sample buffer
  3. Frameshift mutation
  4. Forgot to turn the gel on
  5. Too short elongation time
  6. Forgot template
  7. Multiple annealing sites
  8. Forgot marker
  9. Didn't mix properly
  10. No band in the positive control
  11. Wrong salt/buffer concentration
  12. Primer insertion
  13. Ran gel backwards
  14. Ran gel too long
  15. Prepared agarose gel with dH2O
  16. Band in the negative control
  17. Wrong annealing temp
  18. Wrong primers
  19. Forgot gel stain
  20. Wrong PCR program
  21. Used protein marker as DNA ladder
  22. Spilled the sample
  23. Not enough mastermix
  24. Sample spillover into next lane