WrongannealingtempPrimerinsertionPreparedagarosegel withdH2OToo highvoltage,gelliquifiedMultipleannealingsitesSpilledthesampleForgottemplateToo shortelongationtimeFrameshiftmutationUsed proteinmarker asDNA ladderRan gelbackwardsBand inthenegativecontrolWrongPCRprogramWrongprimersForgotmarkerRan geltoolongDidn't mixwithsamplebufferSamplespilloverinto nextlaneNotenoughmastermixWrongsalt/bufferconcentrationForgotgelstainForgot toturn thegel onNo bandin thepositivecontrolDidn'tmixproperlyWrongannealingtempPrimerinsertionPreparedagarosegel withdH2OToo highvoltage,gelliquifiedMultipleannealingsitesSpilledthesampleForgottemplateToo shortelongationtimeFrameshiftmutationUsed proteinmarker asDNA ladderRan gelbackwardsBand inthenegativecontrolWrongPCRprogramWrongprimersForgotmarkerRan geltoolongDidn't mixwithsamplebufferSamplespilloverinto nextlaneNotenoughmastermixWrongsalt/bufferconcentrationForgotgelstainForgot toturn thegel onNo bandin thepositivecontrolDidn'tmixproperly

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Wrong annealing temp
  2. Primer insertion
  3. Prepared agarose gel with dH2O
  4. Too high voltage, gel liquified
  5. Multiple annealing sites
  6. Spilled the sample
  7. Forgot template
  8. Too short elongation time
  9. Frameshift mutation
  10. Used protein marker as DNA ladder
  11. Ran gel backwards
  12. Band in the negative control
  13. Wrong PCR program
  14. Wrong primers
  15. Forgot marker
  16. Ran gel too long
  17. Didn't mix with sample buffer
  18. Sample spillover into next lane
  19. Not enough mastermix
  20. Wrong salt/buffer concentration
  21. Forgot gel stain
  22. Forgot to turn the gel on
  23. No band in the positive control
  24. Didn't mix properly