Ran gelbackwardsForgot toturn thegel onToo shortelongationtimeBand inthenegativecontrolPrimerinsertionToo highvoltage,gelliquifiedNo bandin thepositivecontrolWrongannealingtempForgotgelstainWrongPCRprogramDidn't mixwithsamplebufferUsed proteinmarker asDNA ladderSamplespilloverinto nextlaneFrameshiftmutationWrongsalt/bufferconcentrationNotenoughmastermixMultipleannealingsitesPreparedagarosegel withdH2OForgotmarkerRan geltoolongForgottemplateDidn'tmixproperlyWrongprimersSpilledthesampleRan gelbackwardsForgot toturn thegel onToo shortelongationtimeBand inthenegativecontrolPrimerinsertionToo highvoltage,gelliquifiedNo bandin thepositivecontrolWrongannealingtempForgotgelstainWrongPCRprogramDidn't mixwithsamplebufferUsed proteinmarker asDNA ladderSamplespilloverinto nextlaneFrameshiftmutationWrongsalt/bufferconcentrationNotenoughmastermixMultipleannealingsitesPreparedagarosegel withdH2OForgotmarkerRan geltoolongForgottemplateDidn'tmixproperlyWrongprimersSpilledthesample

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Ran gel backwards
  2. Forgot to turn the gel on
  3. Too short elongation time
  4. Band in the negative control
  5. Primer insertion
  6. Too high voltage, gel liquified
  7. No band in the positive control
  8. Wrong annealing temp
  9. Forgot gel stain
  10. Wrong PCR program
  11. Didn't mix with sample buffer
  12. Used protein marker as DNA ladder
  13. Sample spillover into next lane
  14. Frameshift mutation
  15. Wrong salt/buffer concentration
  16. Not enough mastermix
  17. Multiple annealing sites
  18. Prepared agarose gel with dH2O
  19. Forgot marker
  20. Ran gel too long
  21. Forgot template
  22. Didn't mix properly
  23. Wrong primers
  24. Spilled the sample