Too highvoltage,gelliquifiedUsed proteinmarker asDNA ladderDidn't mixwithsamplebufferRan gelbackwardsForgotgelstainWrongannealingtempWrongsalt/bufferconcentrationDidn'tmixproperlyBand inthenegativecontrolMultipleannealingsitesSamplespilloverinto nextlanePrimerinsertionForgotmarkerPreparedagarosegel withdH2ONotenoughmastermixForgottemplateRan geltoolongWrongprimersSpilledthesampleForgot toturn thegel onWrongPCRprogramFrameshiftmutationNo bandin thepositivecontrolToo shortelongationtimeToo highvoltage,gelliquifiedUsed proteinmarker asDNA ladderDidn't mixwithsamplebufferRan gelbackwardsForgotgelstainWrongannealingtempWrongsalt/bufferconcentrationDidn'tmixproperlyBand inthenegativecontrolMultipleannealingsitesSamplespilloverinto nextlanePrimerinsertionForgotmarkerPreparedagarosegel withdH2ONotenoughmastermixForgottemplateRan geltoolongWrongprimersSpilledthesampleForgot toturn thegel onWrongPCRprogramFrameshiftmutationNo bandin thepositivecontrolToo shortelongationtime

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Too high voltage, gel liquified
  2. Used protein marker as DNA ladder
  3. Didn't mix with sample buffer
  4. Ran gel backwards
  5. Forgot gel stain
  6. Wrong annealing temp
  7. Wrong salt/buffer concentration
  8. Didn't mix properly
  9. Band in the negative control
  10. Multiple annealing sites
  11. Sample spillover into next lane
  12. Primer insertion
  13. Forgot marker
  14. Prepared agarose gel with dH2O
  15. Not enough mastermix
  16. Forgot template
  17. Ran gel too long
  18. Wrong primers
  19. Spilled the sample
  20. Forgot to turn the gel on
  21. Wrong PCR program
  22. Frameshift mutation
  23. No band in the positive control
  24. Too short elongation time