ForgotgelstainPreparedagarosegel withdH2OWrongsalt/bufferconcentrationToo shortelongationtimePrimerinsertionForgottemplateSamplespilloverinto nextlaneNo bandin thepositivecontrolDidn'tmixproperlyWrongannealingtempUsed proteinmarker asDNA ladderSpilledthesampleDidn't mixwithsamplebufferWrongprimersRan gelbackwardsWrongPCRprogramFrameshiftmutationRan geltoolongNotenoughmastermixBand inthenegativecontrolToo highvoltage,gelliquifiedForgotmarkerForgot toturn thegel onMultipleannealingsitesForgotgelstainPreparedagarosegel withdH2OWrongsalt/bufferconcentrationToo shortelongationtimePrimerinsertionForgottemplateSamplespilloverinto nextlaneNo bandin thepositivecontrolDidn'tmixproperlyWrongannealingtempUsed proteinmarker asDNA ladderSpilledthesampleDidn't mixwithsamplebufferWrongprimersRan gelbackwardsWrongPCRprogramFrameshiftmutationRan geltoolongNotenoughmastermixBand inthenegativecontrolToo highvoltage,gelliquifiedForgotmarkerForgot toturn thegel onMultipleannealingsites

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Forgot gel stain
  2. Prepared agarose gel with dH2O
  3. Wrong salt/buffer concentration
  4. Too short elongation time
  5. Primer insertion
  6. Forgot template
  7. Sample spillover into next lane
  8. No band in the positive control
  9. Didn't mix properly
  10. Wrong annealing temp
  11. Used protein marker as DNA ladder
  12. Spilled the sample
  13. Didn't mix with sample buffer
  14. Wrong primers
  15. Ran gel backwards
  16. Wrong PCR program
  17. Frameshift mutation
  18. Ran gel too long
  19. Not enough mastermix
  20. Band in the negative control
  21. Too high voltage, gel liquified
  22. Forgot marker
  23. Forgot to turn the gel on
  24. Multiple annealing sites