MultipleannealingsitesForgotgelstainForgot toturn thegel onToo highvoltage,gelliquifiedToo shortelongationtimePreparedagarosegel withdH2ORan gelbackwardsWrongprimersSpilledthesampleUsed proteinmarker asDNA ladderBand inthenegativecontrolForgotmarkerWrongannealingtempDidn't mixwithsamplebufferPrimerinsertionFrameshiftmutationNotenoughmastermixWrongsalt/bufferconcentrationNo bandin thepositivecontrolSamplespilloverinto nextlaneRan geltoolongDidn'tmixproperlyForgottemplateWrongPCRprogramMultipleannealingsitesForgotgelstainForgot toturn thegel onToo highvoltage,gelliquifiedToo shortelongationtimePreparedagarosegel withdH2ORan gelbackwardsWrongprimersSpilledthesampleUsed proteinmarker asDNA ladderBand inthenegativecontrolForgotmarkerWrongannealingtempDidn't mixwithsamplebufferPrimerinsertionFrameshiftmutationNotenoughmastermixWrongsalt/bufferconcentrationNo bandin thepositivecontrolSamplespilloverinto nextlaneRan geltoolongDidn'tmixproperlyForgottemplateWrongPCRprogram

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Multiple annealing sites
  2. Forgot gel stain
  3. Forgot to turn the gel on
  4. Too high voltage, gel liquified
  5. Too short elongation time
  6. Prepared agarose gel with dH2O
  7. Ran gel backwards
  8. Wrong primers
  9. Spilled the sample
  10. Used protein marker as DNA ladder
  11. Band in the negative control
  12. Forgot marker
  13. Wrong annealing temp
  14. Didn't mix with sample buffer
  15. Primer insertion
  16. Frameshift mutation
  17. Not enough mastermix
  18. Wrong salt/buffer concentration
  19. No band in the positive control
  20. Sample spillover into next lane
  21. Ran gel too long
  22. Didn't mix properly
  23. Forgot template
  24. Wrong PCR program