Didn't mixwithsamplebufferUsed proteinmarker asDNA ladderWrongsalt/bufferconcentrationWrongPCRprogramForgot toturn thegel onPreparedagarosegel withdH2ONotenoughmastermixToo shortelongationtimeFrameshiftmutationSamplespilloverinto nextlaneNo bandin thepositivecontrolSpilledthesampleDidn'tmixproperlyPrimerinsertionRan geltoolongWrongannealingtempToo highvoltage,gelliquifiedWrongprimersForgottemplateRan gelbackwardsBand inthenegativecontrolForgotgelstainMultipleannealingsitesForgotmarkerDidn't mixwithsamplebufferUsed proteinmarker asDNA ladderWrongsalt/bufferconcentrationWrongPCRprogramForgot toturn thegel onPreparedagarosegel withdH2ONotenoughmastermixToo shortelongationtimeFrameshiftmutationSamplespilloverinto nextlaneNo bandin thepositivecontrolSpilledthesampleDidn'tmixproperlyPrimerinsertionRan geltoolongWrongannealingtempToo highvoltage,gelliquifiedWrongprimersForgottemplateRan gelbackwardsBand inthenegativecontrolForgotgelstainMultipleannealingsitesForgotmarker

PCR/Gelelectrophoresis Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Didn't mix with sample buffer
  2. Used protein marker as DNA ladder
  3. Wrong salt/buffer concentration
  4. Wrong PCR program
  5. Forgot to turn the gel on
  6. Prepared agarose gel with dH2O
  7. Not enough mastermix
  8. Too short elongation time
  9. Frameshift mutation
  10. Sample spillover into next lane
  11. No band in the positive control
  12. Spilled the sample
  13. Didn't mix properly
  14. Primer insertion
  15. Ran gel too long
  16. Wrong annealing temp
  17. Too high voltage, gel liquified
  18. Wrong primers
  19. Forgot template
  20. Ran gel backwards
  21. Band in the negative control
  22. Forgot gel stain
  23. Multiple annealing sites
  24. Forgot marker