QuestionedlifedecisionsForgot towarmTMBReferencerow hashighestresponseDidn't makeenoughblockingbufferNoresponsein positivecontrolNoliquidatortipsDroppeda plateIncubatedtrimer for 1hr59 minutesinstead of 2hoursWent homebeforerememberingto coat platesovernightWantedto dieDroppedentirestack ofplatesNegativecontrollooks likepeeMasterdilutionplatesevaporatedEverythinglooks likepee afterH2SO4AddedTMB butsawnothingSPANKEDTHEPLATESForgot tochangeLiquidatordispensevolumeAddedwrongsecondaryantibodyRandomwell withhigh titersGot bubblesEVERYWHEREAspirated airintomultichannelpipetteWasheddilutionplatesPlatewasherbrokeLosttrack ofdilutionsQuestionedlifedecisionsForgot towarmTMBReferencerow hashighestresponseDidn't makeenoughblockingbufferNoresponsein positivecontrolNoliquidatortipsDroppeda plateIncubatedtrimer for 1hr59 minutesinstead of 2hoursWent homebeforerememberingto coat platesovernightWantedto dieDroppedentirestack ofplatesNegativecontrollooks likepeeMasterdilutionplatesevaporatedEverythinglooks likepee afterH2SO4AddedTMB butsawnothingSPANKEDTHEPLATESForgot tochangeLiquidatordispensevolumeAddedwrongsecondaryantibodyRandomwell withhigh titersGot bubblesEVERYWHEREAspirated airintomultichannelpipetteWasheddilutionplatesPlatewasherbrokeLosttrack ofdilutions

ELISA BINGO - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Questioned life decisions
  2. Forgot to warm TMB
  3. Reference row has highest response
  4. Didn't make enough blocking buffer
  5. No response in positive control
  6. No liquidator tips
  7. Dropped a plate
  8. Incubated trimer for 1hr 59 minutes instead of 2 hours
  9. Went home before remembering to coat plates overnight
  10. Wanted to die
  11. Dropped entire stack of plates
  12. Negative control looks like pee
  13. Master dilution plates evaporated
  14. Everything looks like pee after H2SO4
  15. Added TMB but saw nothing
  16. SPANKED THE PLATES
  17. Forgot to change Liquidator dispense volume
  18. Added wrong secondary antibody
  19. Random well with high titers
  20. Got bubbles EVERYWHERE
  21. Aspirated air into multichannel pipette
  22. Washed dilution plates
  23. Plate washer broke
  24. Lost track of dilutions