(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
List the types of fluorescent markers from smallest to largest.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
Arc lamps pass electricity through ______, _______, or _______ vapour
List one advantage and one disadvantage of small organic dyes.
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
Small organic dyes only work with ______, ______ cells.
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
Light passes the objective lens ______ before reaching the observer or camera.
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
List one benefit of using a deep neural net to reconstruct images.
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
Quantum dots can be approximated as _______.
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
List two reasons why numerical aperture is important for fluorescence microscopy.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List one way fluorescent proteins are better than quantum dots, and one way they are not.
