(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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How should light sources for fluorescence microscopy be chosen?
Quantum dots can be approximated as _______.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List one way fluorescent proteins are better than quantum dots, and one way they are not.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
List two ways photobleaching can be slowed down.
List two reasons why numerical aperture is important for fluorescence microscopy.
Light passes the objective lens ______ before reaching the observer or camera.
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
FlAsH and ReAsH are used in conjunction with _______ proteins.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
List the types of fluorescent markers from smallest to largest.
Arc lamps pass electricity through ______, _______, or _______ vapour
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
Small organic dyes only work with ______, ______ cells.
Photobleaching occurs by ______ state fluorophores reacting with ________.