Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.How shouldlight sources forfluorescencemicroscopy bechosen?A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)FlAsH andReAsH areused inconjunctionwith _______proteins.Light passes theobjective lens______ beforereaching theobserver orcamera.Photobleachingoccurs by______ statefluorophoresreacting with________.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?List the typesof fluorescentmarkers fromsmallest tolargest.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List two waysphotobleachingcan be sloweddown.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?Arc lamps passelectricity through______, _______,or _______ vapour_______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]Small organicdyes onlywork with______,______ cells.Quantumdots can beapproximatedas _______.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.How shouldlight sources forfluorescencemicroscopy bechosen?A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)FlAsH andReAsH areused inconjunctionwith _______proteins.Light passes theobjective lens______ beforereaching theobserver orcamera.Photobleachingoccurs by______ statefluorophoresreacting with________.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?List the typesof fluorescentmarkers fromsmallest tolargest.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List two waysphotobleachingcan be sloweddown.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?Arc lamps passelectricity through______, _______,or _______ vapour_______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]Small organicdyes onlywork with______,______ cells.Quantumdots can beapproximatedas _______.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  2. How should light sources for fluorescence microscopy be chosen?
  3. A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
  4. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  5. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  6. FlAsH and ReAsH are used in conjunction with _______ proteins.
  7. Light passes the objective lens ______ before reaching the observer or camera.
  8. Photobleaching occurs by ______ state fluorophores reacting with ________.
  9. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  10. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  11. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  12. List the types of fluorescent markers from smallest to largest.
  13. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  14. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  15. List two ways photobleaching can be slowed down.
  16. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  17. Arc lamps pass electricity through ______, _______, or _______ vapour
  18. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
  19. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  20. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  21. List two reasons why numerical aperture is important for fluorescence microscopy.
  22. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  23. Small organic dyes only work with ______, ______ cells.
  24. Quantum dots can be approximated as _______.