FlAsH andReAsH areused inconjunctionwith _______proteins.List the typesof fluorescentmarkers fromsmallest tolargest.Arc lamps passelectricity through______, _______,or _______ vapourList one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.Light passes theobjective lens______ beforereaching theobserver orcamera.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List oneadvantage andonedisadvantage ofsmall organicdyes.How shouldlight sources forfluorescencemicroscopy bechosen?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?Photobleachingoccurs by______ statefluorophoresreacting with________.List one benefitof using a deepneural net toreconstructimages.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List two waysphotobleachingcan be sloweddown.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.FlAsH andReAsH areused inconjunctionwith _______proteins.List the typesof fluorescentmarkers fromsmallest tolargest.Arc lamps passelectricity through______, _______,or _______ vapourList one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.Light passes theobjective lens______ beforereaching theobserver orcamera.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List oneadvantage andonedisadvantage ofsmall organicdyes.How shouldlight sources forfluorescencemicroscopy bechosen?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?Photobleachingoccurs by______ statefluorophoresreacting with________.List one benefitof using a deepneural net toreconstructimages.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List two waysphotobleachingcan be sloweddown.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. FlAsH and ReAsH are used in conjunction with _______ proteins.
  2. List the types of fluorescent markers from smallest to largest.
  3. Arc lamps pass electricity through ______, _______, or _______ vapour
  4. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  5. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  6. Light passes the objective lens ______ before reaching the observer or camera.
  7. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  8. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  9. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  10. Small organic dyes only work with ______, ______ cells.
  11. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  12. List one advantage and one disadvantage of small organic dyes.
  13. How should light sources for fluorescence microscopy be chosen?
  14. Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
  15. List two reasons why numerical aperture is important for fluorescence microscopy.
  16. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  17. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  18. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  19. Photobleaching occurs by ______ state fluorophores reacting with ________.
  20. List one benefit of using a deep neural net to reconstruct images.
  21. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  22. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  23. List two ways photobleaching can be slowed down.
  24. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.