Light passes theobjective lens______ beforereaching theobserver orcamera.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]FlAsH andReAsH areused inconjunctionwith _______proteins.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.Photobleachingoccurs by______ statefluorophoresreacting with________.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.Small organicdyes onlywork with______,______ cells.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?_______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.List a databasewhereinformation onfluorophores canbe found.List one benefitof using a deepneural net toreconstructimages.List the typesof fluorescentmarkers fromsmallest tolargest.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.Quantumdots can beapproximatedas _______.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?List two waysphotobleachingcan be sloweddown.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Light passes theobjective lens______ beforereaching theobserver orcamera.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]FlAsH andReAsH areused inconjunctionwith _______proteins.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.Photobleachingoccurs by______ statefluorophoresreacting with________.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.Small organicdyes onlywork with______,______ cells.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?_______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.List a databasewhereinformation onfluorophores canbe found.List one benefitof using a deepneural net toreconstructimages.List the typesof fluorescentmarkers fromsmallest tolargest.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.Quantumdots can beapproximatedas _______.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?List two waysphotobleachingcan be sloweddown.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Light passes the objective lens ______ before reaching the observer or camera.
  2. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  3. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  4. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  5. FlAsH and ReAsH are used in conjunction with _______ proteins.
  6. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  7. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  8. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  9. Photobleaching occurs by ______ state fluorophores reacting with ________.
  10. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  11. Small organic dyes only work with ______, ______ cells.
  12. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  13. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
  14. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  15. List two reasons why numerical aperture is important for fluorescence microscopy.
  16. List a database where information on fluorophores can be found.
  17. List one benefit of using a deep neural net to reconstruct images.
  18. List the types of fluorescent markers from smallest to largest.
  19. A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
  20. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  21. Quantum dots can be approximated as _______.
  22. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  23. List two ways photobleaching can be slowed down.
  24. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?