(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
Light passes the objective lens ______ before reaching the observer or camera.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
List one benefit of using a deep neural net to reconstruct images.
List a database where information on fluorophores can be found.
List two ways photobleaching can be slowed down.
How should light sources for fluorescence microscopy be chosen?
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
List two reasons why numerical aperture is important for fluorescence microscopy.
List one advantage and one disadvantage of small organic dyes.
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
List one way fluorescent proteins are better than quantum dots, and one way they are not.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
Arc lamps pass electricity through ______, _______, or _______ vapour
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
List the types of fluorescent markers from smallest to largest.
Small organic dyes only work with ______, ______ cells.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
