List the typesof fluorescentmarkers fromsmallest tolargest.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.Arc lamps passelectricity through______, _______,or _______ vapourIn the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List one benefitof using a deepneural net toreconstructimages.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two waysphotobleachingcan be sloweddown.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Small organicdyes onlywork with______,______ cells.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Light passes theobjective lens______ beforereaching theobserver orcamera.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List oneadvantage andonedisadvantage ofsmall organicdyes.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?How shouldlight sources forfluorescencemicroscopy bechosen?What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?List a databasewhereinformation onfluorophores canbe found.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.List the typesof fluorescentmarkers fromsmallest tolargest.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.Arc lamps passelectricity through______, _______,or _______ vapourIn the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List one benefitof using a deepneural net toreconstructimages.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two waysphotobleachingcan be sloweddown.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Small organicdyes onlywork with______,______ cells.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Light passes theobjective lens______ beforereaching theobserver orcamera.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List oneadvantage andonedisadvantage ofsmall organicdyes.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?How shouldlight sources forfluorescencemicroscopy bechosen?What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?List a databasewhereinformation onfluorophores canbe found.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. List the types of fluorescent markers from smallest to largest.
  2. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
  3. Arc lamps pass electricity through ______, _______, or _______ vapour
  4. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  5. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  6. List one benefit of using a deep neural net to reconstruct images.
  7. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  8. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
  9. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  10. List two ways photobleaching can be slowed down.
  11. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  12. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  13. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  14. Small organic dyes only work with ______, ______ cells.
  15. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  16. Light passes the objective lens ______ before reaching the observer or camera.
  17. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  18. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  19. List one advantage and one disadvantage of small organic dyes.
  20. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  21. How should light sources for fluorescence microscopy be chosen?
  22. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  23. List a database where information on fluorophores can be found.
  24. List two reasons why numerical aperture is important for fluorescence microscopy.