Photobleachingoccurs by______ statefluorophoresreacting with________.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Light passes theobjective lens______ beforereaching theobserver orcamera.List oneadvantage andonedisadvantage ofsmall organicdyes.How shouldlight sources forfluorescencemicroscopy bechosen?What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?List one benefitof using a deepneural net toreconstructimages.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)Small organicdyes onlywork with______,______ cells.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.FlAsH andReAsH areused inconjunctionwith _______proteins.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Arc lamps passelectricity through______, _______,or _______ vapourList two waysphotobleachingcan be sloweddown.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List the typesof fluorescentmarkers fromsmallest tolargest.List a databasewhereinformation onfluorophores canbe found.Photobleachingoccurs by______ statefluorophoresreacting with________.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Light passes theobjective lens______ beforereaching theobserver orcamera.List oneadvantage andonedisadvantage ofsmall organicdyes.How shouldlight sources forfluorescencemicroscopy bechosen?What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?List one benefitof using a deepneural net toreconstructimages.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)Small organicdyes onlywork with______,______ cells.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.FlAsH andReAsH areused inconjunctionwith _______proteins.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Arc lamps passelectricity through______, _______,or _______ vapourList two waysphotobleachingcan be sloweddown.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List the typesof fluorescentmarkers fromsmallest tolargest.List a databasewhereinformation onfluorophores canbe found.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Photobleaching occurs by ______ state fluorophores reacting with ________.
  2. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  3. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  4. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
  5. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  6. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  7. Light passes the objective lens ______ before reaching the observer or camera.
  8. List one advantage and one disadvantage of small organic dyes.
  9. How should light sources for fluorescence microscopy be chosen?
  10. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  11. List one benefit of using a deep neural net to reconstruct images.
  12. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  13. Small organic dyes only work with ______, ______ cells.
  14. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  15. FlAsH and ReAsH are used in conjunction with _______ proteins.
  16. List two reasons why numerical aperture is important for fluorescence microscopy.
  17. Arc lamps pass electricity through ______, _______, or _______ vapour
  18. List two ways photobleaching can be slowed down.
  19. A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
  20. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  21. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  22. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  23. List the types of fluorescent markers from smallest to largest.
  24. List a database where information on fluorophores can be found.