(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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FlAsH and ReAsH are used in conjunction with _______ proteins.
List the types of fluorescent markers from smallest to largest.
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
Light passes the objective lens ______ before reaching the observer or camera.
List one advantage and one disadvantage of small organic dyes.
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
How should light sources for fluorescence microscopy be chosen?
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
Quantum dots can be approximated as _______.
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List one benefit of using a deep neural net to reconstruct images.
Small organic dyes only work with ______, ______ cells.
List one way fluorescent proteins are better than quantum dots, and one way they are not.
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
List a database where information on fluorophores can be found.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
List two ways photobleaching can be slowed down.
Photobleaching occurs by ______ state fluorophores reacting with ________.
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
