(Print) Use this randomly generated list as your call list when playing the game. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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T-Which of the nucleotides below is found in DNA but not RNA?
T-Thymine
R-What shape is the MIT adapter?
R-Y
M-What is the purpose of ethanol in a bead purification?
M-Removes salt/MM/carrier over and precipitates DNA
A-What is the purpose of the RBC?
A-Index and identify individual samples
S-How many colors does HiSeq have?
S-4
M-Technology used for measuring DNA using PCR.
M-qPCR
A-Why is enzyme treatment sometimes required to purify fungal or bacterial DNA, but not DNA from tissue culture cells?
A-Fungi and bacteria can have tough cell walls
T-Who is the inventor of first generation sequencing?
T-Frederick Sanger
A-Which type of cell is older: Procaryotic or eucaryotic?
A-Procaryotic cells
T-How does the library bind to the flow cell?
T-P5/P7
M-What enzyme adds a 5’phosphate?
M-T4 PNK
M-What is the purpose of beads in a bead purification?
M-Binds DNA
T-How does the Klenow fragment in A-tailing differ from End Repair?
T-Lacks exonuclease activity
T-What is the purpose of Pippin Enrichment?
T-Removes inhibitory library and primer dimers in samples to improve clustering efficiency on NovaSeq
T-Who is the inventor of PCR?
T-Kary Mullis
A-What’s the passing CD on NovaSeq?
A-There is none
A-What is the purpose of DSB in bead purification?
A-DNA is eluted in aqueous solution
S-How many amplicons are in the SNP tracer pool?
S-48
R-ribosomal rna is a component of ribosomes, which are the sites of
R-Translation
T-The primary function of DNAse I is to
T-Digest_DNA
T-What types of molecules does molecular biology primarily deal with?
T-Nucleic acids and proteins
T-What is the purpose of NaOH for sequencing prep?
T-Denatures double stranded DNA (we sequence single stranded DNA)
R-What was the early work on genes referred to?
R-Transmission genetics
M-What are the three termination codons?
M-UAA__UAG__UGA
R-If a poly-T or poly-U resin is incubated with purified total RNA, what type of RNA will make up the majority of material that hybridizes to the beads?
R-mRNA
A-How does the Pippin purify?
A-By electrophoresis
T-How does EDTA function after Ligation?
T-Inhibits reaction, chelates Mg2+
S-What is the directionality of DNA?
S-5 to 3
M-What are the 3 steps of PCR?
M-Denaturation, Annealing, Extension
M-What is the primary advantage of silver stain over other staining methods?
M-Better_sensitivity
S-How much of DNA codes for proteins?
S-1%
T-How does quant-it work?
T-Fluorescently binds double stranded DNA
R-What is the primary advantage of silver stain over other staining methods?
R-Better_sensitivity
S-What is the volume of EDTA added at Ligation?
S-5ul
S-If you are attempting to resolve DNA fragments that range from 100 bp to 500 bp, what concentration of agarose gel would provide the best fragment separation?
S-5%_Gel
S-How many uL’s of DSB/TW are dispensed into -LELU?
S-35ul
R-Buffer Additives such as Formamide and Urea serve what purpose?
R-Denaturing agents
R-Which type of RNA binds individual amino acids and recognizes the matching codons?
R-tRNA
R-___ RNA forms part of the cell structure that provides the site where proteins are synthesized
R-Ribosomal
A-Free!
M-What do each of the MSDS colors mean - blue, red, yellow, and white?
M-Health, flammability, reactivity, other/corrosive
M-What does Western blot target?
M-Protein
A-DNA wrapped around a histone core
A-Nucleosome
R-An ɑ-helix is an example of _______ protein structure.
R-Secondary
T-Where is the DNA in an eucaryotic cells?
T-Within a nucleus
A-What does Northern blot target?
A-RNA
M-Pyrimidine dimers are caused by:
M-Ultraviolet_light
S-How many colors does NovaSeq have?
S-2
A-In a capillary electrophoresis system, which of these particles will migrate most rapidly through the capillary?
A-small_and _negativity_charged
S-What components are needed for ligation to occur?
S-5’P and 3’A
T-How does picogreen bind DNA?
T-Intercalates, and minor groove binder
A-What does Southern blot target?
A-DNA
M-What does STAR stand for?
M-Specific Target Amplification Reaction
S-How much dsb/TW is dispensed into the -SLIB2?
S-25ul
S-How many uL’s of DSB/TW are dispensed into the Long/Short plates?
S-45ul
A-Free!
T-Which amino acids have a pyruvate synthetic precursor?
T-Alanine_ Valine_Leucine
T-Which amino acids are aromatic?
T-phenylalanine, tyrosine, tryptophan
S-What is the directionality of the complementary strand?
S-3 to 5
M-What is the orientation of DNA?
M-Antiparallel
S-What percentage of ethanol do we use for wash plates during KF steps?
S-80%
R-What is the quantitative measurement of Signatera?
R-MTM/mL
R-Where is cfDNA isolated from?
R-Plasma in blood
A-A technology used to determine the sequence of DNA or RNA to study genetic variation associated with diseases or other biological phenomena 1
A-Next Generation Sequencing
S-How many amplicons are in the bespoke primer pool?
S-16
R-What is PhiX?
R-Bacteriophage (balanced base pattern), for sequencing QC
A-The primary function of DNAse I is to
A-Digest_DNA
S-How many hydrogen bonds are in G-C?
S-3
R-A glycoprotein is a protein that is _________ with __________.
R-Conjugated_sugars
M-What factor determines the concentration of agarose gel used for band separation?
M-
Size_in_bp
M-Hybrid discipline that combines elements of genetics and biochemistry. It is the study of gene structure and function at the molecular level.
M-What is Molecular biology?
