Lots ofnonspecificstainingForgotsecondaryonlycontrolGotbubbleswhenmountingAntibodyisn’tcompatibleSpilled orran out ofreagenthalfwayUsedwrongmountingmediaStained10+ slidesat onceHydrophobicdam flew offSuper highbackgroundor autofluorescenceForgotwhichslide waswhichForgotto addDAPINailpolishleakedForgotscale baron imagesUsedwrongantibodydilutionsForgotantigenretrievalStainingworked firsttime but nowdoesn’t workUsedwrongantibodiesForgothumiditychamberStainingdidn’tworkHydrophobicdam leakedForgotsomethingwhile addingAB in thecold roomSectionsdriedoutBoiledsections toomuch duringantigenretrievalAddedprimary ABto secondaryonlyLots ofnonspecificstainingForgotsecondaryonlycontrolGotbubbleswhenmountingAntibodyisn’tcompatibleSpilled orran out ofreagenthalfwayUsedwrongmountingmediaStained10+ slidesat onceHydrophobicdam flew offSuper highbackgroundor autofluorescenceForgotwhichslide waswhichForgotto addDAPINailpolishleakedForgotscale baron imagesUsedwrongantibodydilutionsForgotantigenretrievalStainingworked firsttime but nowdoesn’t workUsedwrongantibodiesForgothumiditychamberStainingdidn’tworkHydrophobicdam leakedForgotsomethingwhile addingAB in thecold roomSectionsdriedoutBoiledsections toomuch duringantigenretrievalAddedprimary ABto secondaryonly

IF Staining Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
  1. Lots of non specific staining
  2. Forgot secondary only control
  3. Got bubbles when mounting
  4. Antibody isn’t compatible
  5. Spilled or ran out of reagent halfway
  6. Used wrong mounting media
  7. Stained 10+ slides at once
  8. Hydrophobic dam flew off
  9. Super high background or auto fluorescence
  10. Forgot which slide was which
  11. Forgot to add DAPI
  12. Nail polish leaked
  13. Forgot scale bar on images
  14. Used wrong antibody dilutions
  15. Forgot antigen retrieval
  16. Staining worked first time but now doesn’t work
  17. Used wrong antibodies
  18. Forgot humidity chamber
  19. Staining didn’t work
  20. Hydrophobic dam leaked
  21. Forgot something while adding AB in the cold room
  22. Sections dried out
  23. Boiled sections too much during antigen retrieval
  24. Added primary AB to secondary only