Stainingworked firsttime but nowdoesn’t workAntibodyisn’tcompatibleForgotsecondaryonlycontrolUsedwrongantibodiesHydrophobicdam flew offLots ofnonspecificstainingAddedprimary ABto secondaryonlyUsedwrongmountingmediaForgothumiditychamberNailpolishleakedHydrophobicdam leakedSuper highbackgroundor autofluorescenceGotbubbleswhenmountingStainingdidn’tworkForgotscale baron imagesForgotto addDAPIBoiledsections toomuch duringantigenretrievalStained10+ slidesat onceForgotantigenretrievalUsedwrongantibodydilutionsSectionsdriedoutSpilled orran out ofreagenthalfwayForgotwhichslide waswhichForgotsomethingwhile addingAB in thecold roomStainingworked firsttime but nowdoesn’t workAntibodyisn’tcompatibleForgotsecondaryonlycontrolUsedwrongantibodiesHydrophobicdam flew offLots ofnonspecificstainingAddedprimary ABto secondaryonlyUsedwrongmountingmediaForgothumiditychamberNailpolishleakedHydrophobicdam leakedSuper highbackgroundor autofluorescenceGotbubbleswhenmountingStainingdidn’tworkForgotscale baron imagesForgotto addDAPIBoiledsections toomuch duringantigenretrievalStained10+ slidesat onceForgotantigenretrievalUsedwrongantibodydilutionsSectionsdriedoutSpilled orran out ofreagenthalfwayForgotwhichslide waswhichForgotsomethingwhile addingAB in thecold room

IF Staining Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Staining worked first time but now doesn’t work
  2. Antibody isn’t compatible
  3. Forgot secondary only control
  4. Used wrong antibodies
  5. Hydrophobic dam flew off
  6. Lots of non specific staining
  7. Added primary AB to secondary only
  8. Used wrong mounting media
  9. Forgot humidity chamber
  10. Nail polish leaked
  11. Hydrophobic dam leaked
  12. Super high background or auto fluorescence
  13. Got bubbles when mounting
  14. Staining didn’t work
  15. Forgot scale bar on images
  16. Forgot to add DAPI
  17. Boiled sections too much during antigen retrieval
  18. Stained 10+ slides at once
  19. Forgot antigen retrieval
  20. Used wrong antibody dilutions
  21. Sections dried out
  22. Spilled or ran out of reagent halfway
  23. Forgot which slide was which
  24. Forgot something while adding AB in the cold room