Forgotsomethingwhile addingAB in thecold roomStainingworked firsttime but nowdoesn’t workForgotto addDAPIForgotscale baron imagesGotbubbleswhenmountingForgotsecondaryonlycontrolAntibodyisn’tcompatibleForgothumiditychamberHydrophobicdam flew offNailpolishleakedSuper highbackgroundor autofluorescenceAddedprimary ABto secondaryonlyUsedwrongantibodiesStained10+ slidesat onceForgotwhichslide waswhichStainingdidn’tworkLots ofnonspecificstainingUsedwrongmountingmediaSectionsdriedoutSpilled orran out ofreagenthalfwayForgotantigenretrievalHydrophobicdam leakedUsedwrongantibodydilutionsBoiledsections toomuch duringantigenretrievalForgotsomethingwhile addingAB in thecold roomStainingworked firsttime but nowdoesn’t workForgotto addDAPIForgotscale baron imagesGotbubbleswhenmountingForgotsecondaryonlycontrolAntibodyisn’tcompatibleForgothumiditychamberHydrophobicdam flew offNailpolishleakedSuper highbackgroundor autofluorescenceAddedprimary ABto secondaryonlyUsedwrongantibodiesStained10+ slidesat onceForgotwhichslide waswhichStainingdidn’tworkLots ofnonspecificstainingUsedwrongmountingmediaSectionsdriedoutSpilled orran out ofreagenthalfwayForgotantigenretrievalHydrophobicdam leakedUsedwrongantibodydilutionsBoiledsections toomuch duringantigenretrieval

IF Staining Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Forgot something while adding AB in the cold room
  2. Staining worked first time but now doesn’t work
  3. Forgot to add DAPI
  4. Forgot scale bar on images
  5. Got bubbles when mounting
  6. Forgot secondary only control
  7. Antibody isn’t compatible
  8. Forgot humidity chamber
  9. Hydrophobic dam flew off
  10. Nail polish leaked
  11. Super high background or auto fluorescence
  12. Added primary AB to secondary only
  13. Used wrong antibodies
  14. Stained 10+ slides at once
  15. Forgot which slide was which
  16. Staining didn’t work
  17. Lots of non specific staining
  18. Used wrong mounting media
  19. Sections dried out
  20. Spilled or ran out of reagent halfway
  21. Forgot antigen retrieval
  22. Hydrophobic dam leaked
  23. Used wrong antibody dilutions
  24. Boiled sections too much during antigen retrieval