This bingo card has a free space and 24 words: How should light sources for fluorescence microscopy be chosen?, List one advantage and one disadvantage of small organic dyes., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., Small organic dyes only work with ______, ______ cells., List a database where information on fluorophores can be found., When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., Quantum dots can be approximated as _______., Photobleaching occurs by ______ state fluorophores reacting with ________., Light passes the objective lens ______ before reaching the observer or camera., Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., List two reasons why numerical aperture is important for fluorescence microscopy., List the types of fluorescent markers from smallest to largest., What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, List two ways photobleaching can be slowed down., Arc lamps pass electricity through ______, _______, or _______ vapour, (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], In the Rayleigh criterion formula, n refers to the index of refraction of which medium? and DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?.
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