What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Small organicdyes onlywork with______,______ cells.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.List the typesof fluorescentmarkers fromsmallest tolargest.List two waysphotobleachingcan be sloweddown.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?How shouldlight sources forfluorescencemicroscopy bechosen?Arc lamps passelectricity through______, _______,or _______ vapour(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List a databasewhereinformation onfluorophores canbe found.List oneadvantage andonedisadvantage ofsmall organicdyes.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Light passes theobjective lens______ beforereaching theobserver orcamera.Quantumdots can beapproximatedas _______.Photobleachingoccurs by______ statefluorophoresreacting with________.Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?Small organicdyes onlywork with______,______ cells.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.List the typesof fluorescentmarkers fromsmallest tolargest.List two waysphotobleachingcan be sloweddown.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?How shouldlight sources forfluorescencemicroscopy bechosen?Arc lamps passelectricity through______, _______,or _______ vapour(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List a databasewhereinformation onfluorophores canbe found.List oneadvantage andonedisadvantage ofsmall organicdyes.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Light passes theobjective lens______ beforereaching theobserver orcamera.Quantumdots can beapproximatedas _______.Photobleachingoccurs by______ statefluorophoresreacting with________.Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  2. Small organic dyes only work with ______, ______ cells.
  3. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
  4. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
  5. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  6. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  7. List two reasons why numerical aperture is important for fluorescence microscopy.
  8. List the types of fluorescent markers from smallest to largest.
  9. List two ways photobleaching can be slowed down.
  10. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  11. How should light sources for fluorescence microscopy be chosen?
  12. Arc lamps pass electricity through ______, _______, or _______ vapour
  13. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  14. List a database where information on fluorophores can be found.
  15. List one advantage and one disadvantage of small organic dyes.
  16. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  17. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  18. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  19. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  20. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  21. Light passes the objective lens ______ before reaching the observer or camera.
  22. Quantum dots can be approximated as _______.
  23. Photobleaching occurs by ______ state fluorophores reacting with ________.
  24. Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.