(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
How should light sources for fluorescence microscopy be chosen?
Arc lamps pass electricity through ______, _______, or _______ vapour
List a database where information on fluorophores can be found.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
List one advantage and one disadvantage of small organic dyes.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
Small organic dyes only work with ______, ______ cells.
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
Photobleaching occurs by ______ state fluorophores reacting with ________.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List two reasons why numerical aperture is important for fluorescence microscopy.
Light passes the objective lens ______ before reaching the observer or camera.
List the types of fluorescent markers from smallest to largest.
List two ways photobleaching can be slowed down.
Quantum dots can be approximated as _______.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
