This bingo card has a free space and 24 words: DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, List one benefit of using a deep neural net to reconstruct images., Small organic dyes only work with ______, ______ cells., Light passes the objective lens ______ before reaching the observer or camera., Photobleaching occurs by ______ state fluorophores reacting with ________., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), How should light sources for fluorescence microscopy be chosen?, Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., List one advantage and one disadvantage of small organic dyes., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., Arc lamps pass electricity through ______, _______, or _______ vapour, What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, Quantum dots can be approximated as _______., (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, List two reasons why numerical aperture is important for fluorescence microscopy., List two ways photobleaching can be slowed down., List a database where information on fluorophores can be found. and A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample..
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