(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
List a database where information on fluorophores can be found.
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
How should light sources for fluorescence microscopy be chosen?
Small organic dyes only work with ______, ______ cells.
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
List one advantage and one disadvantage of small organic dyes.
Arc lamps pass electricity through ______, _______, or _______ vapour
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
Light passes the objective lens ______ before reaching the observer or camera.
List two reasons why numerical aperture is important for fluorescence microscopy.
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
List two ways photobleaching can be slowed down.
List one benefit of using a deep neural net to reconstruct images.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
Quantum dots can be approximated as _______.
Photobleaching occurs by ______ state fluorophores reacting with ________.
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
