This bingo card has a free space and 24 words: DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, How should light sources for fluorescence microscopy be chosen?, Small organic dyes only work with ______, ______ cells., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., List one benefit of using a deep neural net to reconstruct images., List one way fluorescent proteins are better than quantum dots, and one way they are not., List two ways photobleaching can be slowed down., Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., List one advantage and one disadvantage of small organic dyes., Quantum dots can be approximated as _______., What was the first chemical to be known to have fluorescent properties, and who discovered this fact?, List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, List the types of fluorescent markers from smallest to largest., List a database where information on fluorophores can be found., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., Light passes the objective lens ______ before reaching the observer or camera. and When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?.
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