(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
Quantum dots can be approximated as _______.
List a database where information on fluorophores can be found.
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
List one advantage and one disadvantage of small organic dyes.
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
List two ways photobleaching can be slowed down.
Small organic dyes only work with ______, ______ cells.
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
List one way fluorescent proteins are better than quantum dots, and one way they are not.
List one benefit of using a deep neural net to reconstruct images.
How should light sources for fluorescence microscopy be chosen?
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List the types of fluorescent markers from smallest to largest.
Light passes the objective lens ______ before reaching the observer or camera.
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
