This bingo card has a free space and 24 words: Photobleaching occurs by ______ state fluorophores reacting with ________., What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, List two reasons why numerical aperture is important for fluorescence microscopy., Small organic dyes only work with ______, ______ cells., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, List a database where information on fluorophores can be found., When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, Quantum dots can be approximated as _______., Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, What was the first chemical to be known to have fluorescent properties, and who discovered this fact?, List the types of fluorescent markers from smallest to largest., Arc lamps pass electricity through ______, _______, or _______ vapour, List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., FlAsH and ReAsH are used in conjunction with _______ proteins., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., List one way fluorescent proteins are better than quantum dots, and one way they are not., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., List two ways photobleaching can be slowed down., The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, How should light sources for fluorescence microscopy be chosen?, DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, Dichroic mirrors reflect _____ wavelengths and transmit ______ ones. and List one advantage and one disadvantage of small organic dyes..
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