When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?How shouldlight sources forfluorescencemicroscopy bechosen?In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List oneadvantage andonedisadvantage ofsmall organicdyes.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List two waysphotobleachingcan be sloweddown.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Arc lamps passelectricity through______, _______,or _______ vapourList the typesof fluorescentmarkers fromsmallest tolargest.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?FlAsH andReAsH areused inconjunctionwith _______proteins.Quantumdots can beapproximatedas _______.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List a databasewhereinformation onfluorophores canbe found.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?Photobleachingoccurs by______ statefluorophoresreacting with________._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?How shouldlight sources forfluorescencemicroscopy bechosen?In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List oneadvantage andonedisadvantage ofsmall organicdyes.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?List two waysphotobleachingcan be sloweddown.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Arc lamps passelectricity through______, _______,or _______ vapourList the typesof fluorescentmarkers fromsmallest tolargest.List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?FlAsH andReAsH areused inconjunctionwith _______proteins.Quantumdots can beapproximatedas _______.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.List a databasewhereinformation onfluorophores canbe found.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?Photobleachingoccurs by______ statefluorophoresreacting with________._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  2. How should light sources for fluorescence microscopy be chosen?
  3. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
  4. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  5. List one advantage and one disadvantage of small organic dyes.
  6. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  7. List two ways photobleaching can be slowed down.
  8. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  9. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  10. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  11. Arc lamps pass electricity through ______, _______, or _______ vapour
  12. List the types of fluorescent markers from smallest to largest.
  13. List two reasons why numerical aperture is important for fluorescence microscopy.
  14. Small organic dyes only work with ______, ______ cells.
  15. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  16. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  17. FlAsH and ReAsH are used in conjunction with _______ proteins.
  18. Quantum dots can be approximated as _______.
  19. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  20. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  21. List a database where information on fluorophores can be found.
  22. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  23. Photobleaching occurs by ______ state fluorophores reacting with ________.
  24. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.