(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
FlAsH and ReAsH are used in conjunction with _______ proteins.
Small organic dyes only work with ______, ______ cells.
List two reasons why numerical aperture is important for fluorescence microscopy.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
List the types of fluorescent markers from smallest to largest.
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
Photobleaching occurs by ______ state fluorophores reacting with ________.
List two ways photobleaching can be slowed down.
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
How should light sources for fluorescence microscopy be chosen?
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
Quantum dots can be approximated as _______.
Arc lamps pass electricity through ______, _______, or _______ vapour
List one way fluorescent proteins are better than quantum dots, and one way they are not.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List one advantage and one disadvantage of small organic dyes.
List a database where information on fluorophores can be found.
