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This bingo card has a free space and 24 words: Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample., Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?, The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, Light passes the objective lens ______ before reaching the observer or camera., What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, List a database where information on fluorophores can be found., List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, How should light sources for fluorescence microscopy be chosen?, Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., List two ways photobleaching can be slowed down., FlAsH and ReAsH are used in conjunction with _______ proteins., List one way fluorescent proteins are better than quantum dots, and one way they are not., What was the first chemical to be known to have fluorescent properties, and who discovered this fact?, Photobleaching occurs by ______ state fluorophores reacting with ________., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), Small organic dyes only work with ______, ______ cells., List one advantage and one disadvantage of small organic dyes., List the types of fluorescent markers from smallest to largest., List two reasons why numerical aperture is important for fluorescence microscopy. and (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease].

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