(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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List one way fluorescent proteins are better than quantum dots, and one way they are not.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
FlAsH and ReAsH are used in conjunction with _______ proteins.
How should light sources for fluorescence microscopy be chosen?
List two reasons why numerical aperture is important for fluorescence microscopy.
Light passes the objective lens ______ before reaching the observer or camera.
Small organic dyes only work with ______, ______ cells.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
Photobleaching occurs by ______ state fluorophores reacting with ________.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
List two ways photobleaching can be slowed down.
List the types of fluorescent markers from smallest to largest.
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
List a database where information on fluorophores can be found.
List one advantage and one disadvantage of small organic dyes.
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
