(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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List a database where information on fluorophores can be found.
List one advantage and one disadvantage of small organic dyes.
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
Photobleaching occurs by ______ state fluorophores reacting with ________.
List two reasons why numerical aperture is important for fluorescence microscopy.
List two ways photobleaching can be slowed down.
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
FlAsH and ReAsH are used in conjunction with _______ proteins.
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
List the types of fluorescent markers from smallest to largest.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
How should light sources for fluorescence microscopy be chosen?
In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
Light passes the objective lens ______ before reaching the observer or camera.
List one way fluorescent proteins are better than quantum dots, and one way they are not.
Small organic dyes only work with ______, ______ cells.
