Photobleachingoccurs by______ statefluorophoresreacting with________.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.List a databasewhereinformation onfluorophores canbe found.List two waysphotobleachingcan be sloweddown.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List the typesof fluorescentmarkers fromsmallest tolargest.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?How shouldlight sources forfluorescencemicroscopy bechosen?Light passes theobjective lens______ beforereaching theobserver orcamera.List oneadvantage andonedisadvantage ofsmall organicdyes.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.FlAsH andReAsH areused inconjunctionwith _______proteins.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.Photobleachingoccurs by______ statefluorophoresreacting with________.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.List a databasewhereinformation onfluorophores canbe found.List two waysphotobleachingcan be sloweddown.List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.List the typesof fluorescentmarkers fromsmallest tolargest.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?How shouldlight sources forfluorescencemicroscopy bechosen?Light passes theobjective lens______ beforereaching theobserver orcamera.List oneadvantage andonedisadvantage ofsmall organicdyes.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi.FlAsH andReAsH areused inconjunctionwith _______proteins.What was the firstchemical to beknown to havefluorescentproperties, andwho discoveredthis fact?The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?Small organicdyes onlywork with______,______ cells.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List two reasonswhy numericalaperture isimportant forfluorescencemicroscopy.Super resolutionmicroscopy ispromising because itallows us to surpasstwo physical limits offluorescentmicroscopes. What arethe names of theselimits?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Photobleaching occurs by ______ state fluorophores reacting with ________.
  2. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  3. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  4. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  5. List a database where information on fluorophores can be found.
  6. List two ways photobleaching can be slowed down.
  7. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  8. List the types of fluorescent markers from smallest to largest.
  9. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  10. How should light sources for fluorescence microscopy be chosen?
  11. Light passes the objective lens ______ before reaching the observer or camera.
  12. List one advantage and one disadvantage of small organic dyes.
  13. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
  14. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  15. FlAsH and ReAsH are used in conjunction with _______ proteins.
  16. What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
  17. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  18. Small organic dyes only work with ______, ______ cells.
  19. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  20. List two reasons why numerical aperture is important for fluorescence microscopy.
  21. Super resolution microscopy is promising because it allows us to surpass two physical limits of fluorescent microscopes. What are the names of these limits?
  22. Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
  23. A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
  24. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.