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This bingo card has a free space and 24 words: Quantum dots can be approximated as _______., When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample., FlAsH and ReAsH are used in conjunction with _______ proteins., How should light sources for fluorescence microscopy be chosen?, Light passes the objective lens ______ before reaching the observer or camera., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., List one advantage and one disadvantage of small organic dyes., Arc lamps pass electricity through ______, _______, or _______ vapour, (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], List one way fluorescent proteins are better than quantum dots, and one way they are not., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., List two ways photobleaching can be slowed down., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, What was the first chemical to be known to have fluorescent properties, and who discovered this fact?, The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, Small organic dyes only work with ______, ______ cells., List a database where information on fluorophores can be found. and List two reasons why numerical aperture is important for fluorescence microscopy..

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