(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
List one advantage and one disadvantage of small organic dyes.
DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
Small organic dyes only work with ______, ______ cells.
How should light sources for fluorescence microscopy be chosen?
When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
List two reasons why numerical aperture is important for fluorescence microscopy.
In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
_______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
(MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]
What was the first chemical to be known to have fluorescent properties, and who discovered this fact?
Quantum dots can be approximated as _______.
(MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
List one way fluorescent proteins are better than quantum dots, and one way they are not.
Arc lamps pass electricity through ______, _______, or _______ vapour
The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
List a database where information on fluorophores can be found.
List two ways photobleaching can be slowed down.
FlAsH and ReAsH are used in conjunction with _______ proteins.
Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
Light passes the objective lens ______ before reaching the observer or camera.
A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
