Photobleachingoccurs by______ statefluorophoresreacting with________.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Quantumdots can beapproximatedas _______.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.Small organicdyes onlywork with______,______ cells.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List oneadvantage andonedisadvantage ofsmall organicdyes.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List two waysphotobleachingcan be sloweddown.List one benefitof using a deepneural net toreconstructimages.Light passes theobjective lens______ beforereaching theobserver orcamera.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.FlAsH andReAsH areused inconjunctionwith _______proteins.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.List the typesof fluorescentmarkers fromsmallest tolargest.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]Photobleachingoccurs by______ statefluorophoresreacting with________.Between these threeevents, which is theslowest? The events:absorption ofphotons, internal lossof energy in anexcited state,emission of photons.A microscope with anepi-illuminativestructure is amicroscope that isresponsible for both______ andillumination of theobserved sample.Quantumdots can beapproximatedas _______.What colourwavelength is thehardest to directlyengineer from agreen fluorescentprotein?List one advantage ofusing fluorophores toknock out proteinsover “traditional”genetics-basedmethods like RNAi._______ filtercubes allow forlarge signals,while ______filter cubes givehigh specificity.Small organicdyes onlywork with______,______ cells.Dichroic mirrorsreflect _____wavelengthsand transmit______ ones.DsRed-E5 is aprotein timer that, asit matures undergoesa colour change tored. What is the initialcolour of thisfluorophore?List oneadvantage andonedisadvantage ofsmall organicdyes.(MCQ) Which of thefollowing applicationsis FRAP not typicallyused for? (Diffusioninformation, proteinconformationalchanges, protein-protein bindinginformation)List one wayfluorescentproteins are betterthan quantumdots, and one waythey are not.The extinctioncoefficient, whichrelates quantum yieldand brightness, is aproportionalityconstant in whichother physical law?List two waysphotobleachingcan be sloweddown.List one benefitof using a deepneural net toreconstructimages.Light passes theobjective lens______ beforereaching theobserver orcamera.In a triplet state,electrons are notinvolved in theirbonding, and as aresult, their spinstates can be_______ to eachother.FlAsH andReAsH areused inconjunctionwith _______proteins.In the Rayleighcriterion formula, nrefers to the indexof refraction ofwhich medium?When utilising FRET togain biological data,we are appealing tothe precise, sensitiverelationship betweendistance and anotherphysical quantity. Whatis this quantity?Quantum yield is theratio of the ______ ofradiative events tothe sum of the _____of radiative and non-radiative events.List the typesof fluorescentmarkers fromsmallest tolargest.(MCQ) A higherdegree of conjugationin a fluorophore willcause thewavelength ofemitted light to_____. [Increase,Decrease]

Untitled Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Photobleaching occurs by ______ state fluorophores reacting with ________.
  2. Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons.
  3. A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample.
  4. Quantum dots can be approximated as _______.
  5. What colour wavelength is the hardest to directly engineer from a green fluorescent protein?
  6. List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi.
  7. _______ filter cubes allow for large signals, while ______ filter cubes give high specificity.
  8. Small organic dyes only work with ______, ______ cells.
  9. Dichroic mirrors reflect _____ wavelengths and transmit ______ ones.
  10. DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?
  11. List one advantage and one disadvantage of small organic dyes.
  12. (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information)
  13. List one way fluorescent proteins are better than quantum dots, and one way they are not.
  14. The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?
  15. List two ways photobleaching can be slowed down.
  16. List one benefit of using a deep neural net to reconstruct images.
  17. Light passes the objective lens ______ before reaching the observer or camera.
  18. In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other.
  19. FlAsH and ReAsH are used in conjunction with _______ proteins.
  20. In the Rayleigh criterion formula, n refers to the index of refraction of which medium?
  21. When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?
  22. Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events.
  23. List the types of fluorescent markers from smallest to largest.
  24. (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease]