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This bingo card has a free space and 24 words: What colour wavelength is the hardest to directly engineer from a green fluorescent protein?, Dichroic mirrors reflect _____ wavelengths and transmit ______ ones., _______ filter cubes allow for large signals, while ______ filter cubes give high specificity., Quantum yield is the ratio of the ______ of radiative events to the sum of the _____ of radiative and non-radiative events., Light passes the objective lens ______ before reaching the observer or camera., FlAsH and ReAsH are used in conjunction with _______ proteins., (MCQ) Which of the following applications is FRAP not typically used for? (Diffusion information, protein conformational changes, protein-protein binding information), When utilising FRET to gain biological data, we are appealing to the precise, sensitive relationship between distance and another physical quantity. What is this quantity?, Between these three events, which is the slowest? The events: absorption of photons, internal loss of energy in an excited state, emission of photons., Small organic dyes only work with ______, ______ cells., List one benefit of using a deep neural net to reconstruct images., In a triplet state, electrons are not involved in their bonding, and as a result, their spin states can be _______ to each other., A microscope with an epi-illuminative structure is a microscope that is responsible for both ______ and illumination of the observed sample., (MCQ) A higher degree of conjugation in a fluorophore will cause the wavelength of emitted light to _____. [Increase, Decrease], In the Rayleigh criterion formula, n refers to the index of refraction of which medium?, List two ways photobleaching can be slowed down., DsRed-E5 is a protein timer that, as it matures undergoes a colour change to red. What is the initial colour of this fluorophore?, List one advantage of using fluorophores to knock out proteins over “traditional” genetics-based methods like RNAi., List one advantage and one disadvantage of small organic dyes., Quantum dots can be approximated as _______., List the types of fluorescent markers from smallest to largest., The extinction coefficient, which relates quantum yield and brightness, is a proportionality constant in which other physical law?, Photobleaching occurs by ______ state fluorophores reacting with ________. and List one way fluorescent proteins are better than quantum dots, and one way they are not..

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