Free!The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Encapsulationis method ofphysicallimmobilization.Inspissation isthe proces ofthickening bydehydration ininspissator.We canmonitoreautoclaves byheat sensitivechemicals.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.Filtration isphysicalmethod ofsterilization.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.Tyndallizationmay fail withspores of someanaerobes andthermophiles.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Heatsterilization canbe performedwith dry ormoist heat.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.Immobilizationis a methodfor recoveringenzymes.The air in theautoclavechamber isevacuated andfilled withsaturated steam.Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.Steam at 100oC contains 7xas muchenergy aswater at 100oC.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Moist heat kills-microorganismsby denaturingand coagulatingproteins.UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.Free!The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Encapsulationis method ofphysicallimmobilization.Inspissation isthe proces ofthickening bydehydration ininspissator.We canmonitoreautoclaves byheat sensitivechemicals.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.Filtration isphysicalmethod ofsterilization.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.Tyndallizationmay fail withspores of someanaerobes andthermophiles.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Heatsterilization canbe performedwith dry ormoist heat.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.Immobilizationis a methodfor recoveringenzymes.The air in theautoclavechamber isevacuated andfilled withsaturated steam.Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.Steam at 100oC contains 7xas muchenergy aswater at 100oC.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Moist heat kills-microorganismsby denaturingand coagulatingproteins.UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.

Bioprocess engineering Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Free!
  2. The usual temperature for autoclave is 121 oC at exposure time of 15-20 minutes.
  3. Steam sterilizers autoclaves use pressurized steam heat to kill any microbial life that may be present on a contaminated load.
  4. Main use of filtration is for heat labile substances like antibiotics, vitamins, amino acids, proteins and many gases.
  5. Encapsulation is method of physicall immobilization.
  6. Inspissation is the proces of thickening by dehydration in inspissator.
  7. We can monitore autoclaves by heat sensitive chemicals.
  8. Sterilization can be defined as any proces that effectively kills or eliminates transmissible agents (fungi, bacteria, viruses, prions).
  9. Due to its reversibility and simplicity, the most favored method of immobilization is physical adsorption.
  10. Filtration is physical method of sterilization.
  11. Immobilization of biological material is related to attachment of a compund of interest to/within a support or matrix.
  12. Tyndallization may fail with spores of some anaerobes and thermophiles.
  13. We distinguish gravity method, vacuum and liquid method in kinds of autoclaves.
  14. Heat sterilization can be performed with dry or moist heat.
  15. Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
  16. Filtration removes bacteria but viruses and some small bacteria may pass through.
  17. Both ethanol and isopropanol are widely used disinfectants, usually at concentration of about 70%.
  18. The action of heat in sterilization will be due to induction of lethal events mediated through the action of water and oxygen.
  19. Immobilization is a method for recovering enzymes.
  20. The air in the autoclave chamber is evacuated and filled with saturated steam.
  21. Thermal Death Time – a minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment.
  22. Steam at 100 oC contains 7x as much energy as water at 100 oC.
  23. Advantages of heat sterilization are good penetrability and non-corosive nature.
  24. DISINFECTANT – products used to kill microorganisms on inanimate objects or surfaces.
  25. Moist heat kills - microorganisms by denaturing and coagulating proteins.
  26. UV light is generally used in irradiation of air in certain areas e.g. operating rooms and laboratories.