Inspissation isthe proces ofthickening bydehydration ininspissator.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.Immobilizationis a methodfor recoveringenzymes.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.Tyndallizationmay fail withspores of someanaerobes andthermophiles.Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Filtration isphysicalmethod ofsterilization.Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.Heatsterilization canbe performedwith dry ormoist heat.Encapsulationis method ofphysicallimmobilization.We canmonitoreautoclaves byheat sensitivechemicals.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Free!Steam at 100oC contains 7xas muchenergy aswater at 100oC.The air in theautoclavechamber isevacuated andfilled withsaturated steam.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Moist heat kills-microorganismsby denaturingand coagulatingproteins.Inspissation isthe proces ofthickening bydehydration ininspissator.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.Immobilizationis a methodfor recoveringenzymes.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.Tyndallizationmay fail withspores of someanaerobes andthermophiles.Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Filtration isphysicalmethod ofsterilization.Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.Heatsterilization canbe performedwith dry ormoist heat.Encapsulationis method ofphysicallimmobilization.We canmonitoreautoclaves byheat sensitivechemicals.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Free!Steam at 100oC contains 7xas muchenergy aswater at 100oC.The air in theautoclavechamber isevacuated andfilled withsaturated steam.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Moist heat kills-microorganismsby denaturingand coagulatingproteins.

Bioprocess engineering Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Inspissation is the proces of thickening by dehydration in inspissator.
  2. Advantages of heat sterilization are good penetrability and non-corosive nature.
  3. Immobilization is a method for recovering enzymes.
  4. Sterilization can be defined as any proces that effectively kills or eliminates transmissible agents (fungi, bacteria, viruses, prions).
  5. UV light is generally used in irradiation of air in certain areas e.g. operating rooms and laboratories.
  6. Steam sterilizers autoclaves use pressurized steam heat to kill any microbial life that may be present on a contaminated load.
  7. Tyndallization may fail with spores of some anaerobes and thermophiles.
  8. Due to its reversibility and simplicity, the most favored method of immobilization is physical adsorption.
  9. The usual temperature for autoclave is 121 oC at exposure time of 15-20 minutes.
  10. Filtration removes bacteria but viruses and some small bacteria may pass through.
  11. Filtration is physical method of sterilization.
  12. Thermal Death Time – a minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment.
  13. Heat sterilization can be performed with dry or moist heat.
  14. Encapsulation is method of physicall immobilization.
  15. We can monitore autoclaves by heat sensitive chemicals.
  16. The action of heat in sterilization will be due to induction of lethal events mediated through the action of water and oxygen.
  17. We distinguish gravity method, vacuum and liquid method in kinds of autoclaves.
  18. Immobilization of biological material is related to attachment of a compund of interest to/within a support or matrix.
  19. DISINFECTANT – products used to kill microorganisms on inanimate objects or surfaces.
  20. Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
  21. Free!
  22. Steam at 100 oC contains 7x as much energy as water at 100 oC.
  23. The air in the autoclave chamber is evacuated and filled with saturated steam.
  24. Both ethanol and isopropanol are widely used disinfectants, usually at concentration of about 70%.
  25. Main use of filtration is for heat labile substances like antibiotics, vitamins, amino acids, proteins and many gases.
  26. Moist heat kills - microorganisms by denaturing and coagulating proteins.