Filtration isphysicalmethod ofsterilization.Encapsulationis method ofphysicallimmobilization.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.The air in theautoclavechamber isevacuated andfilled withsaturated steam.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Moist heat kills-microorganismsby denaturingand coagulatingproteins.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Immobilizationis a methodfor recoveringenzymes.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Steam at 100oC contains 7xas muchenergy aswater at 100oC.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.Tyndallizationmay fail withspores of someanaerobes andthermophiles.Free!Inspissation isthe proces ofthickening bydehydration ininspissator.Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.We canmonitoreautoclaves byheat sensitivechemicals.Heatsterilization canbe performedwith dry ormoist heat.UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.Filtration isphysicalmethod ofsterilization.Encapsulationis method ofphysicallimmobilization.Immobilization ofbiological materialis related toattachment of acompund ofinterest to/within asupport or matrix.Sterilization can bedefined as anyproces thateffectively kills oreliminatestransmissible agents(fungi, bacteria,viruses, prions).Thermal Death Time –a minimum timerequired to kill asuspension oforganisms at apredeterminedtemperature in aspecified environment.The action of heatin sterilization willbe due to inductionof lethal eventsmediated throughthe action of waterand oxygen.The air in theautoclavechamber isevacuated andfilled withsaturated steam.DISINFECTANT –products used tokillmicroorganismson inanimateobjects orsurfaces.Moist heat kills-microorganismsby denaturingand coagulatingproteins.We distinguishgravity method,vacuum andliquid method inkinds ofautoclaves.Both ethanol andisopropanol arewidely useddisinfectants,usually atconcentration ofabout 70%.The usualtemperature forautoclave is 121oC at exposuretime of 15-20minutes.Immobilizationis a methodfor recoveringenzymes.Autoclave is apressurized devicedesigned to heataqueous solutionsabove their boilingpoint at normalatmospheric pressureto achieve sterilization.Main use offiltration is for heatlabile substanceslike antibiotics,vitamins, aminoacids, proteins andmany gases.Steam at 100oC contains 7xas muchenergy aswater at 100oC.Advantages ofheat sterilizationare goodpenetrability andnon-corosivenature.Tyndallizationmay fail withspores of someanaerobes andthermophiles.Free!Inspissation isthe proces ofthickening bydehydration ininspissator.Due to itsreversibility andsimplicity, the mostfavored method ofimmobilization isphysicaladsorption.We canmonitoreautoclaves byheat sensitivechemicals.Heatsterilization canbe performedwith dry ormoist heat.UV light isgenerally used inirradiation of air incertain areas e.g.operating roomsand laboratories.Filtrationremoves bacteriabut viruses andsome smallbacteria maypass through.Steam sterilizersautoclaves usepressurized steamheat to kill anymicrobial life thatmay be present on acontaminated load.

Bioprocess engineering Bingo - Call List

(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.


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  1. Filtration is physical method of sterilization.
  2. Encapsulation is method of physicall immobilization.
  3. Immobilization of biological material is related to attachment of a compund of interest to/within a support or matrix.
  4. Sterilization can be defined as any proces that effectively kills or eliminates transmissible agents (fungi, bacteria, viruses, prions).
  5. Thermal Death Time – a minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment.
  6. The action of heat in sterilization will be due to induction of lethal events mediated through the action of water and oxygen.
  7. The air in the autoclave chamber is evacuated and filled with saturated steam.
  8. DISINFECTANT – products used to kill microorganisms on inanimate objects or surfaces.
  9. Moist heat kills - microorganisms by denaturing and coagulating proteins.
  10. We distinguish gravity method, vacuum and liquid method in kinds of autoclaves.
  11. Both ethanol and isopropanol are widely used disinfectants, usually at concentration of about 70%.
  12. The usual temperature for autoclave is 121 oC at exposure time of 15-20 minutes.
  13. Immobilization is a method for recovering enzymes.
  14. Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
  15. Main use of filtration is for heat labile substances like antibiotics, vitamins, amino acids, proteins and many gases.
  16. Steam at 100 oC contains 7x as much energy as water at 100 oC.
  17. Advantages of heat sterilization are good penetrability and non-corosive nature.
  18. Tyndallization may fail with spores of some anaerobes and thermophiles.
  19. Free!
  20. Inspissation is the proces of thickening by dehydration in inspissator.
  21. Due to its reversibility and simplicity, the most favored method of immobilization is physical adsorption.
  22. We can monitore autoclaves by heat sensitive chemicals.
  23. Heat sterilization can be performed with dry or moist heat.
  24. UV light is generally used in irradiation of air in certain areas e.g. operating rooms and laboratories.
  25. Filtration removes bacteria but viruses and some small bacteria may pass through.
  26. Steam sterilizers autoclaves use pressurized steam heat to kill any microbial life that may be present on a contaminated load.