(Print) Use this randomly generated list as your call list when playing the game. There is no need to say the BINGO column name. Place some kind of mark (like an X, a checkmark, a dot, tally mark, etc) on each cell as you announce it, to keep track. You can also cut out each item, place them in a bag and pull words from the bag.
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Immobilization is a method for recovering enzymes.
Thermal Death Time – a minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment.
Sterilization can be defined as any proces that effectively kills or eliminates transmissible agents (fungi, bacteria, viruses, prions).
Immobilization of biological material is related to attachment of a compund of interest to/within a support or matrix.
Moist heat kills - microorganisms by denaturing and coagulating proteins.
We distinguish gravity method, vacuum and liquid method in kinds of autoclaves.
Encapsulation is method of physicall immobilization.
Advantages of heat sterilization are good penetrability and non-corosive nature.
Steam at 100 oC contains 7x as much energy as water at 100 oC.
Free!
Filtration removes bacteria but viruses and some small bacteria may pass through.
The air in the autoclave chamber is evacuated and filled with saturated steam.
DISINFECTANT – products used to kill microorganisms on inanimate objects or surfaces.
Filtration is physical method of sterilization.
Tyndallization may fail with spores of some anaerobes and thermophiles.
Steam sterilizers autoclaves use pressurized steam heat to kill any microbial life that may be present on a contaminated load.
We can monitore autoclaves by heat sensitive chemicals.
Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
Both ethanol and isopropanol are widely used disinfectants, usually at concentration of about 70%.
Due to its reversibility and simplicity, the most favored method of immobilization is physical adsorption.
The action of heat in sterilization will be due to induction of lethal events mediated through the action of water and oxygen.
UV light is generally used in irradiation of air in certain areas e.g. operating rooms and laboratories.
Inspissation is the proces of thickening by dehydration in inspissator.
Main use of filtration is for heat labile substances like antibiotics, vitamins, amino acids, proteins and many gases.
The usual temperature for autoclave is 121 oC at exposure time of 15-20 minutes.
Heat sterilization can be performed with dry or moist heat.
